ISG15和OAS1在奶牛早期妊娠阶段外周血中的表达规律

doi:10.11843/j.issn.0366-6964.2015.01.010

Abstract

Abstract:

This study aimed to investigate mRNA transcriptions of interferon stimulated genes ISG15 and OAS1 in the peripheral blood of Chinese Holstein dairy cows during early pregnancy，by which we hope to provide an insight into their applications in bovine early pregnancy diagnosis.A total of 27 heifers following spontaneous estrus(n=10) or oestrus synchronisation(n=17) were used for the study.Peripheral blood were collected at 0，14，18，21 and 28 d post artificial insemination(AI)；subsets of peripheral blood leukocytes(lymphocyte，monocyte，and natural killer cell) were isolated at 0，18 and 21 d；Real-time PCR was employed to detect the relative mRNA transcriptions of ISG15 and OAS1 in the whole white blood cells(WBC) and/or the leukocyte subpopulations.The results showed that bovine ISG15 and OAS1 in the peripheral blood of pregnant cows were significantly elevated versus non-pregnant cows at 18 d(P<0.05)；On 18 d post AI，ISG15 was up-regulated in pregnant cows with the average fold change(FC) of 3.92，whereas it was down-regulated in non-pregnant cows with the average FC of 1.46；OAS1 was similarly up-regulated(FC=3.49) in pregnant cows and down-regulated(FC=1.28) in non-pregnant cows.The relative transcriptions of ISG15 and OAS1 in pregnant cows following spontaneous estrus were significantly higher than those in non-pregnant cows at 18 d post AI；although the tendency in cows following oestrus synchronisation was the same，the difference was not statistically significant.Further analysis in the subpopulations of leukocytes showed that OAS1 in lymphocytes of pregnant cows was significantly elevated(+2.14)，whereas depressed in non-pregnant cows (-1.80) at 18 d post AI(P<0.05).The results indicated that regulations of ISG15 in peripheral blood of pregnant cows were more obvious than OAS1 at 18 d after AI；differential expressions of ISG15 and OAS1 between pregnant cows and non-pregnant cows were more obvious in cows following spontaneous estrus.In conclusion，ISG15 transcriptions in peripheral WBC could be a better indicator for bovine early pregnancy diagnosis(18 d post AI)，especially in cows following spontaneous estrus.Gene expression analysis of bovine ISG15 and OAS1 in the leukocyte subsets has provided additional insight into their roles during early maternal recognition of pregnancy.

CLC Number:

S823.9⁺1.2

CHENG Lei，WANG Ding-fa，LIU Xiao-hua，XIANG Min，LING Ming-hu，HU Xiu-zhong，XIA Yu. Study on ISG15 and OAS1 Transcriptions in Peripheral Blood of Dairy Cows during Early Pregnancy[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 77-84.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2015.01.010

https://www.xmsyxb.com/EN/Y2015/V46/I1/77

References

［1］NAKAO T，SUGIHASHI A，KAWATA K，et al.Milk progesterone levels in cows with normal or prolonged estrous cycles，referenced to an early pregnancy diagnosis［J］.Jpn J Vet Sci，1983，45：495-499.
［2］MORTON J M，WYNN P C.Assessing ovulation detection performance in commercial dairy herds using progesterone concentrations from limited numbers of strategically collected milk samples［J］.J Dairy Sci，2010，93：3019-3030.
［3］ZOLI A P，GUIBAULT L A，DELAHAUT P，et al.Radioimmunoassay of a bovine pregnancy-associated glycoprotein in serum：its application for pregnancy diagnosis［J］.Biol Reprod，1992，46：83-92.
［4］FRICKE P M.Scanning the future - ultrasonography as a reproductive management tool for dairy cattle［J］.J Dairy Sci，2002，85：1918-1926.
［5］LUCY M C，MCDOUGALL S，NATION D P.The use of hormonal treatments to improve the reproductive performance of lactating dairy cows in feedlot or pasture-based management systems［J］.Anim Reprod Sci，2004，82-83：495-512.
［6］SPENCER T E，BAZER F W.Conceptus signals for establishment and maintenance of pregnancy［J］.Reprod Biol Endocrinol，2004，2：49-63.
［7］BOTT R C，ASHLEY R L，HENKES L E，et al.Uterine vein infusion of interferon tau(IFNT) extends luteal life span in ewes［J］.Biol Reprod，2010，82(4)：725-735.
［8］GREEN J C，OKAMURA C S，POOCK S E，et al.Measurement of interferontau(IFN-tau) stimulated gene expression in blood leukocytes for pregnancy diagnosis within 18-20 d after insemination in dairy cattle［J］.Anim Reprod Sci，2010，121：24-33.
［9］OLIVEIRA J F，HENKES L E，ASHLEY R L，et al.Expression of interferon(IFN)-stimulated genes in extrauterine tissues during early pregnancy in sheep is the consequence of endocrine IFN-tau release from the uterine vein［J］.Endocrinology，2008，149(3)：1252-1259.
［10］GIFFORD C A，RACICOT K，CLARK D S，et al.Regulation of interferon-stimulated genes in peripheral blood leukocytes in pregnant and bred，Nonpregnant dairy cows［J］.J Dairy Sci，2007，90：274-280.
［11］HAN H，AUSTIN K J，REMPEL L A，et al.Low blood ISG15 mRNA and progesterone levels are predictive of non-pregnant dairy cows［J］.J Endocrinol，2006，191：505-512.
［12］谢胜松，李新云，苏立杰，等.一种简易快速鉴定动物组织总RNA质量的方法［J］.农业生物技术学报，2009，2：282-287.
XIE S S,LI X Y ,SU L J,et al.A facile，rapid and effective method for RNA quality determination［J］.Journal of Agricultural Biotechnology,2009，2：282-287.(in Chinese)
［13］LIVAK K J，SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method［J］.Methods，2001，25：402-408.
［14］BURTON J L，MADSEN S A，CHANG L C，et al.Gene expression signatures in neutrophils exposed to glucocorticoids：a new paradigm to help explain “neutrophil dysfunction” in parturient dairy cows［J］.Vet Immunol Immunopath，2005，105：197-219.
［15］SUKUMARAN B，CARLYON J A，CAI J L，et al.Early transcriptional response of human neutrophils to Anaplasma phagocytophilum infection［J］.Infect Immun，2005，73：8089-8099.
［16］OTT T L，GIFFORD C A.Effects of early conceptus signals on circulating immune cells：lessons from domestic ruminants［J］.Am J Reprod Immunol，2010，64：245-254.
［17］BUSCH D C，WILSON D J，SCHAFER D J，et al.Comparison of progestin-based estrus synchronization protocols before fixed-time artificial insemination on pregnancy rate in beef heifers［J］.J Anim Sci，2007，85：1933-1939.
［18］COLAZO M G，KASTELIC J P，SMALL J A，et al.Resynchronization of estrus in beef cattle：Ovarian function，estrus and fertility following progestin treatment and treatments to synchronize ovarian follicular development and estrus［J］.Can Vet J，2007；48：49-56.
［19］OTT T L，GIFFORD C A.Effects of early conceptus signals on circulating immune cells：lessons from domestic ruminants［J］.Am J Reprod Immunol，2010，64(4)：245-54.
［20］OLIVEIRA L J，MANSOURRI-ATTIA N，FAHEY A G，et al.Characterization of the profile of the bovine endometrium during the oestrous cycle and early pregnancy［J］.PLoS ONE，2013，8(10)：e75571.
［21］SAMUEL C E.Antiviral actions of interferons［J］.Clin Microbiol Rev，2001，14：778-809.
［22］TOCHIZAWA S，OHMOTO Y，MORI T.A novel modification of a flow cytometric assay of phosphorylated STAT1 in whole blood lymphocytes for rapid detection of interferon-alpha signal in vivo［J］.J Immunol Methods，2006，313：29-37.
［23］KIZAKI K，SHICHIJO-KIZAKI A，FURUSAWA T，et al.Differential neutrophil gene expression in early bovine pregnancy［J］.Reprod Biol Endocrinol，2013，11：6.

[1]	ZHAO Shanjiang, SHUAI Zhiqiang, PANG Yunwei, HAO Haisheng, ZOU Huiying, DU Weihua, ZHAO Xueming, ZHU Huabin. Effect of Age at First Pregnancy on Milk Production and Reproductive Performance During the First 2 Lactations in Holstein Dairy Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 732-742.
[2]	ZHAO Chang, ZHANG Jiang, BAI Yunlong, SUN Shuhan, SONG Yuxi, XIA Cheng. Analysis of Serum Differential Proteins in Cows with Inactive Ovaries Based on iTRAQ Technology [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 972-982.
[3]	LI Xiao-jun, LI Xin, CHEN Xiao-li, ZHAO Yi-qiang, LU Yong-qiang, WANG Dong. The Transcription of Pld6 during Spermiogenesis and the Analysis of Its Protein Sequence in Dairy Cows [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(12): 2632-2640.
[4]	GUO Yan-sheng, TAO Jin-zhong. Selection of Milk Biomarkers of Pregnancy Recognition in Dairy Cows Based on LC-Q/TOF-MS Metabolomics [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(8): 1633-1641.
[5]	LIU Ying-chun, CHEN Yu-meng, HAN Zhe-xian, GAO Feng, ZHOU Huan-min. Induction and Identification of Neural Cell from Mammary Stem Cells in Holstein Cows and the Effect of Prolactin on Its Proliferative Activity [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(3): 542-548.
[6]	XU Chao, LI Feng, HAO Hai-sheng, LIU Yan, ZHAO Xue-ming, PANG Yun-wei, ZHU Hua-bin, DU Wei-hua. Sex-Related Genes Expression during Development of the Early Bovine Fetuses [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(10): 1892-1901.
[7]	ZHAO Yan-kun, SHAO Wei, WANG Li-wen, YU Xiong. Effect of Co-culturing with Umbilical Cord Mesenchymal Stem Cells on Milk Fat Synthesis and Expression of Key Genes in Bovine Mammary Gland Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(5): 846-853.
[8]	LIU Xiao-qian，LIU Ying, RONG Chao，WANG Kun，XIAO Hang，ZHANG Yuan-shu. Expression Localization of Angiotensin Converting Enzyme 2 (ACE 2) in Dairy Cow Mammary Gland and Its Anti-inflammation Effect [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(3): 552-560.
[9]	LI Xiao-jun，WANG Zhen-ling，CHEN Xiao-li，KOU Hong-xiang，LI Xiu-ling，WANG Yan，WANG Dong. Study Progress on the Rule of Body Temperature and Its Application in Reproduction of Dairy Cattle [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2331-2341.
[10]	WANG Xiao, XIE Xiao-li, WANG Sheng, QIAN Meng-ying, MA Pei-pei, ZHANG Yuan, SUN Dong-xiao, LIU Jian-feng, DING Xiang-dong, JIANG Li, WANG Ya-chun, ZHANG Yi, ZHANG Sheng-li, ZHANG Qin,YU Ying. Genome-wide Association Study for Mastitis Susceptibility and Resistance in Chinese Holsteins [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1907-1912.
[11]	ZHANG Mei-rong, CHEN Dan, LIAO Xiang-xiang, MAO Yong-jiang, LIU Kun, CHEN Liang, WANG Xing-long, YANG Zhang-ping, YANG Li-guo. Genetic Polymorphism of IL8 Gene and Its Association with Milk Traits and SCS in Holstein Imported from Australia [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 690-696.
[12]	WANG Hao-yu, QIN Tong, HAO Hai-sheng, DU Wei-hua, ZHAO Xue-ming, ZHU Hua-bin. Effects of Insulin on mRNA Expression of Genes Related to Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells Cultured in vitro [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(5): 710-718.

Study on ISG15 and OAS1 Transcriptions in Peripheral Blood of Dairy Cows during Early Pregnancy

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 12

Recommended Articles

Metrics

Comments